Part:BBa_J05503

Sensing Device A (cI) TESTER (Pr+GFP+Prm+RFP)

A debug version of the event sensing and processing system for the iGEM2005 ETH "Counter" project: its purpose is to regulate cI production dependent on an external event. It works somehow like an inverter:

STATE 1: when IPTG is absent, the constitutively produced lacI protein will block the lacI+pL promoter so that no cI is produced. Thus downstream the Pr promoter will be constitutively active, producing GFP (green fluorescence), while Prm+ will have low or no activity (no/low RFP - red fluorescence).

STATE 2: when IPTG is present, it will remove the lacI protein from the lacI+pL promoter and cI is produced. Thus downstream the Pr promoter will be repressed (no GFP, i.e. no green fluorescence) by cI, while Prm+ will be activated, producing RFP (red fluorescence).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1227
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 3982
  Illegal AgeI site found at 4094
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3098