Regulatory
Input sys.

Part:BBa_J05501

Designed by: ETH Zurich iGEM Team 2005   Group: iGEM2005   (2005-09-22)


Sensing Device B (cI+LVA)

The event sensing system for the iGEM2005 ETH "Counter" project: its purpose is to regulate cI+LVA (with degradation tag) production dependent on an external event. It works somehow like an inverter:

STATE 1: when IPTG is absent, the constitutively produced lacI protein will block the lacI+pL promoter so that no cI is produced. Thus downstream (not included in this composite) the Pr promoter will be constitutively active, producing some zinc finger proteins, while Prm+ will have low or no activity.

STATE 2: when IPTG is present, it will remove the lacI protein from the lacI+pL promoter and cI is produced. Thus downstream (not included in this composite) the Pr promoter will be repressed by cI, while Prm+ will be activated, producing some other zinc finger proteins

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/regulatory/uncategorized
Parameters
None