Measurement
ZF test

Part:BBa_J05000

Designed by: NOR-Group   Group: iGEM2005   (2005-08-29)


Zinc Finger Protein Tester

Tester to investigate zinc finger proteins (ZFP) as a negative regulator of expression. Lac promoter controls expression of a ZFP and a reporter (i.e. RFP) to confirm expression. Lambda promoter constitutively expresses GFP unless ZFP binds to the binding site after the lambda promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1116
    Illegal AgeI site found at 1228
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2163


[edit]
Categories
//classic/measurement/uncategorized
Parameters
None