Part:BBa_J04800

J04800 (RevAptRibo) contains a theophylline aptamer upstream of the RBS that should act as a riboswi

Like J04700 and J04900, J04800 contains a promoter, a theophylline aptamer, an 8bp sequence and an RBS, in that order. On either end, J04800 uses strandard Biobrick ends. The aptamer sequence comes from {R. Jenison, S. Gill, A. Pardi, B. Polisky, Science 263, 1426 (1994)}. However, the aptamer sequence is in reverse nucleotide order, hence the name, ReverseAptamerRiboswitch (RevAptRibo). The aptamer portion of the mRNA transcript should alter shape when it binds theophylline. This change in shape may affect expression levels, which would make this a synthetic, theophylline-dependent riboswitch. The Biobrick ends allow for convenient ligation of the riboswitch to a gene of choice.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]