Regulatory
mTCT8-4

Part:BBa_J04705

Designed by: Kristen DeCelle   Group: iGEM2005   (2005-09-11)


Riboswitch designed to turn "ON" a protein

The riboswitch is designed to be linked to an RBS using a 5-8 bp sequence. Once transcribed into RNA, the riboswitch will hopefully turn on whatever protein we want it to. At this point, we hope to turn on mCherry.


Usage and Biology

This Riboswitch's name is mTCT8-4 and was taken directly out of: Robert D. Jenison, Stanley C. Gill, Arthur Pardi, Barry Polisky. "High-Resolution Molecular Discrimination by RNA." Science. Vol. 263: 11 March 1994. The riboswitch is designed to turn on a protein with increasing amounts of theophylline.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/regulatory/uncategorized
Parameters
None