Part:BBa_J04686

Antiswitch repressing production of EYFP ("Off" switch)

The presence of theophylline prevents translation of mRNA transcribed from Biobrick BBa_E0032. The absence of theophylline allows expression of the coding region of BBa_E0032.

Usage and Biology

The antiswitch acts as an allosteric riboregulator, that in the presence of theophylline prevents translation of a target mRNA by attaching 3 bp upstream of the start codon of the target sequence, inhibiting translation of the coding region. In the absence of theophylline, the antiswitch remains free from the mRNA and translation can proceed as normal. The target sequence for this antiswitch is: uagauggugagcaag. The plasmid containing this part is Blue Heron pUCminusMCS, which itself also expresses ampicillin resistance.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 94
  Illegal XbaI site found at 125
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 94
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 94
  Illegal BamHI site found at 55
  Illegal XhoI site found at 227
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 94
  Illegal XbaI site found at 125
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 94
  Illegal XbaI site found at 125
  
• 1000
  COMPATIBLE WITH RFC[1000]