RNA

Part:BBa_J04676

Designed by: Oscar Hernandez and Matt Gemberling   Group: iGEM2005   (2005-09-15)


R0010 with Anti-Sense Theophillline Induced Antiswitch ("Off Switch")

This Promoter and Antiswitch construct is designed so that it can be attached to any form of EYFP or YFP coding region. The purpose of this piece is to regulate the expression of YFP in the presence of theophylline. In this construct, the presence of theophylline causes the antiswitch, which is initially bound up on itself (allowing expression), to release and unfold allowing it to bind to the YFP. This, in turn, halts the expression of YFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 201
    Illegal XhoI site found at 373
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/rna/uncategorized
Parameters
None