Part:BBa_J04606
Antiswitch repressing Biobrick coding sequence at the RBS ("Off" switch)
The presence of theophylline prevents translation of mRNA coding region directly downstream from any Biobrick that contains the BBa_B0034 RBS. This effect is the result of the antiswitch attaching to the B0034 RBS preceding the coding region. The absence of theophylline allows expression of the coding region as usual.
Usage and Biology
The antiswitch acts as an allosteric riboregulator, that in the presence of theophylline prevents translation of a target mRNA by attaching to the RBS of an mRNA transcribed from Biobrick BBa_B0034, inhibiting translation of the coding region. In the absence of theophylline, the antiswitch remains free from the mRNA and translation can proceed as normal. The target sequence for this antiswitch is: gagaaagaggagaaa. The plasmid containing this part is Blue Heron pUCminusMCS, which itself also expresses ampicillin resistance.
When tested, this antiswitch appears to turn the production of YFP "ON" rather than "OFF".
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 94
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 94
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 94
Illegal BamHI site found at 55
Illegal XhoI site found at 124
Illegal XhoI site found at 227 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 94
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 94
- 1000COMPATIBLE WITH RFC[1000]
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