IPTG inducible promoter with RBS
Usage and Biology
R0010 and B0034 will be digested and ligated together in the manner described on the "Registry of Standard Biological Parts" website.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
Contribution made by SHSID 2020
Recombinant FnCpf1 production, purification, and SDS-PAGE analysis
The backbone for BBa_K3521005 was derived from pET-28a vector. The recombinant plasmid was transformed into BL21 (DE3) competent cells and induced with IPTG. After induction, a specific protein band that is consistent with the theoretical molecular weight of FnCpf1 was detected in SDS-PAGE (Figure 2). The recombinant FnCpf1 was successfully purified by Ni-affinity chromatography.