RNA

Part:BBa_J04086

Designed by: Matt Gemberling   Group: iGEM2005   (2005-12-04)


"Off" Antiswitch and target EYFP in the same plasmid

This is an "Off" antiswitch placed into the same plasmid as the target EYFP. Both have the same promoter. This will help to ensure a close ratio of Antiswitch to EYFP mRNA. This part was designed based on Smolke and Bayer (2005) antiswitch S8.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 201
    Illegal XhoI site found at 373
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
//classic/rna/uncategorized
Parameters
None