Reporter

Part:BBa_J04031

Designed by: Kristen DeCelle   Group: iGEM2005   (2005-06-08)


GFP w/LVA tag

BBa_E0040 with an LVA tag added via PCR. BBa_E0040 is GFP(mut3b).


Usage and Biology

Part was obtained by adding, via PCR, an LVA tag modeled off of the one found in E0032. We decided to include the same restriction site found in E0032 immediately before the start of the 11 amino acid LVA tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


[edit]
Categories
//function/reporter
Parameters
None