Intermediate

Part:BBa_J01072

Designed by: Golden Bear   Group: iGEM2005   (2005-11-05)


TEST [pBad/araC][Key2][pTet][Lock2][RFP]

Test for activation of Lock 2 by Key 2. Need a significant increase in RFP expression for desired behavior.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1960
    Illegal AgeI site found at 2072
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1216
    Illegal SapI site found at 961


[edit]
Categories
//classic/intermediate/uncategorized
Parameters
None