Intermediate
Part:BBa_J01071
Designed by: Golden Bear Group: iGEM2005 (2005-11-05)
TEST [pBad/araC][Key2][pTet][Lock1][RFP]
For testing cross activation of Key 2 with Lock 1. Need to have little to no increase in RFP expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1960
Illegal AgeI site found at 2072 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1216
Illegal SapI site found at 961
[edit]
Categories
Parameters
//classic/intermediate/uncategorized
None |