Intermediate
Part:BBa_J01070
Designed by: Golden Bear Group: iGEM2005 (2005-11-05)
TEST [pBad/araC][Key1][pTet][Lock2][RFP]
For testing cross activation of Key 1 with Lock 2. Need to have little to no increase in RFP expression.
Usage and Biology
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Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1284
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1284
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1284
Illegal AgeI site found at 979
Illegal AgeI site found at 1960
Illegal AgeI site found at 2072 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1216
Illegal SapI site found at 961
Functional Parameters
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Categories
Parameters
//classic/intermediate/uncategorized
None |