Intermediate

Part:BBa_J01056

Designed by: Golden Bear   Group: iGEM2005   (2005-11-05)


Non mobilizable TraJF, OnCFP, Key2 = [pBad/araC TraJF][DblTerminator][OnCFP][OnKey2]

-- No description --


Usage and Biology

1 of four first generation main construct pieces for Berkeley iGEM 2005. Construction was abandoned when lock2/key2 activation was found to be too weak

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1281
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3084
    Illegal SapI site found at 961


[edit]
Categories
//classic/intermediate/uncategorized
Parameters
None