Coding

Part:BBa_I769102

Designed by: Mackenize Cowell   Group: iGEM07_Example   (2007-06-27)


Feruloyl-CoA hydratase for vanillin biosynthesis

One of two steps in the bioconversion of ferulic acid to vanillin. The enzyme is from Pseudomonas fluorescens and it does work with E. coli. It requires both ech and fcs for vanillin biosynthesis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 811
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 811
    Illegal NotI site found at 755
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 811
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 811
    Illegal NgoMIV site found at 184
    Illegal NgoMIV site found at 202
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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