Generator

Part:BBa_I766999

Designed by: Jimmy Jie Min Huang   Group: iGEM07_UCSF   (2007-08-16)


YopJ-RR under strong constitutive promotor

This device will express YopJ, a pathogen derived irreversible inhibitor (acetylase) of MapKK Ste7 under the pAdh1 promotor. The effector protein is fused to an RR leucine zipper and will be recruited to elements possessing the matching EE zipper. The effector is fused mCherry (RFP) on the N-terminal.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2752
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2752
    Illegal NotI site found at 3311
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1801
    Illegal BamHI site found at 1642
    Illegal BamHI site found at 3294
    Illegal XhoI site found at 2392
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2752
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2752
    Illegal NgoMIV site found at 224
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 874
    Illegal BsaI.rc site found at 1570
    Illegal SapI.rc site found at 1594


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Parameters
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