Generator
Part:BBa_I766999
Designed by: Jimmy Jie Min Huang Group: iGEM07_UCSF (2007-08-16)
YopJ-RR under strong constitutive promotor
This device will express YopJ, a pathogen derived irreversible inhibitor (acetylase) of MapKK Ste7 under the pAdh1 promotor. The effector protein is fused to an RR leucine zipper and will be recruited to elements possessing the matching EE zipper. The effector is fused mCherry (RFP) on the N-terminal.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2752
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2752
Illegal NotI site found at 3311 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1801
Illegal BamHI site found at 1642
Illegal BamHI site found at 3294
Illegal XhoI site found at 2392 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2752
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2752
Illegal NgoMIV site found at 224 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 874
Illegal BsaI.rc site found at 1570
Illegal SapI.rc site found at 1594
[edit]
Categories
Parameters
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