Generator

Part:BBa_I766997

Designed by: Nili Sommovilla   Group: iGEM07_UCSF   (2007-10-25)

OspF-EE under medium constituitive promoter

OspF-EE under control of a Cyc1 promoter

Usage and Biology

OspF is a bacterial phosphothreonine lyase, apparently specific for MAPKs. It catalyzes the removal of the phosphorylated threonine side chain present in the MAPK activation loop, therefore inactivating the kinase in an irreversible manner. Here, OspF is N-terminally fused to mCherry, and C-terminally to the synthetic leucine zipper EE; being expressed under control of a Cyc1 promoter (constitutive medium level expression).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 784
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 784
    Illegal NotI site found at 1343
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1326
    Illegal XhoI site found at 424
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 784
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 784
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None