Plasmid

Part:BBa_I766994

Designed by: Nili Sommovilla   Group: iGEM07_UCSF   (2007-10-25)


pRS315

shuttle vector bacteria, yeast, amp resistance, LEU2 marker

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1116
    Illegal EcoRI site found at 3178
    Illegal XbaI site found at 3148
    Illegal SpeI site found at 3154
    Illegal PstI site found at 3172
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1116
    Illegal EcoRI site found at 3178
    Illegal SpeI site found at 3154
    Illegal PstI site found at 3172
    Illegal NotI site found at 3140
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1116
    Illegal EcoRI site found at 3178
    Illegal BamHI site found at 3160
    Illegal XhoI site found at 2410
    Illegal XhoI site found at 3211
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1116
    Illegal EcoRI site found at 3178
    Illegal XbaI site found at 3148
    Illegal SpeI site found at 3154
    Illegal PstI site found at 3172
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1116
    Illegal EcoRI site found at 3178
    Illegal XbaI site found at 3148
    Illegal SpeI site found at 3154
    Illegal PstI site found at 3172
    Illegal NgoMIV site found at 2799
    Illegal AgeI site found at 1503
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4584
    Illegal SapI site found at 3501
    Illegal SapI site found at 5605


[edit]
Categories
Parameters
chassis
copies
mcs
origin
resistanceA