Generator

Part:BBa_I766685

Designed by: alexander ng   Group: iGEM07_UCSF   (2007-08-16)

YopJ-EE under inducible promotor

This device will express YopJ, a pathogen derived irreversible inhibitor (acetylase) of MapKK Ste7 under the Fig1 promotor, inducible by activation of the mating pathway. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing a matching RR zipper. The effector is fused mCherry (RFP) on the N terminal.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1370
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1370
    Illegal NotI site found at 2810
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2026
    Illegal BamHI site found at 1867
    Illegal BamHI site found at 2793
    Illegal XhoI site found at 1010
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1370
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1370
    Illegal NgoMIV site found at 172
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1795
    Illegal SapI.rc site found at 1819


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Categories
Parameters
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