Generator

Part:BBa_I766677

Designed by: Lauren Jann   Group: iGEM07_UCSF   (2007-08-16)


YopH-RR under strong constitutive promoter

This device will consititutively express YopH, a pathogen derived reversible inhibitor (phosphatase) of Map Kinase Fus3, under the pAdh promoter. The effector protein is fused to a RR zipper, and will be recruited to elements possessing a matching EE zipper. The effector is fused to mCherry (RFP) on the N-terminus.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2703
    Illegal PstI site found at 1871
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1871
    Illegal NotI site found at 3851
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3834
    Illegal XhoI site found at 1511
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2703
    Illegal PstI site found at 1871
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2703
    Illegal PstI site found at 1871
    Illegal NgoMIV site found at 224
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 874
    Illegal BsaI site found at 3208


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Categories
Parameters
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