Device
Part:BBa_I766676
Designed by: Eric Chou Group: iGEM07_UCSF (2007-08-16)
YopH-EE under weak constitutive promotor
This device will express YopH, a pathogen derived irreversible inhibitor (phosphotase) of MapK Fus3 under the pSte5 promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing the matching EE zipper. The effector is fused mCherry (RFP) on the N-terminal.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 93
Illegal XbaI site found at 1077
Illegal PstI site found at 2392 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2392
Illegal NotI site found at 2951 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2934
Illegal XhoI site found at 2032 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 93
Illegal XbaI site found at 1077
Illegal PstI site found at 2392 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 93
Illegal XbaI site found at 1077
Illegal PstI site found at 2392 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1582
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Categories
Parameters
None |