Device

Part:BBa_I766676

Designed by: Eric Chou   Group: iGEM07_UCSF   (2007-08-16)


YopH-EE under weak constitutive promotor

This device will express YopH, a pathogen derived irreversible inhibitor (phosphotase) of MapK Fus3 under the pSte5 promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing the matching EE zipper. The effector is fused mCherry (RFP) on the N-terminal.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 93
    Illegal XbaI site found at 1077
    Illegal PstI site found at 2392
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2392
    Illegal NotI site found at 2951
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2934
    Illegal XhoI site found at 2032
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 93
    Illegal XbaI site found at 1077
    Illegal PstI site found at 2392
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 93
    Illegal XbaI site found at 1077
    Illegal PstI site found at 2392
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1582


[edit]
Categories
Parameters
None