Generator

Part:BBa_I766673

Designed by: Eric Chou   Group: iGEM07_UCSF   (2007-08-16)

YopH-EE under strong constitutive promotor

This device will express YopH, a pathogen derived irreversible inhibitor (phosphotase) of MapK Fus3 under the pAdh promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing the matching RR zipper. The effector is fused mCherry (RFP) on the N-terminal.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1977
    Illegal PstI site found at 3292
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3292
    Illegal NotI site found at 3851
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3834
    Illegal XhoI site found at 2932
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1977
    Illegal PstI site found at 3292
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1977
    Illegal PstI site found at 3292
    Illegal NgoMIV site found at 224
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 874
    Illegal BsaI site found at 2482


[edit]
Categories
Parameters
None