Device

Part:BBa_I766672

Designed by: Eric Chou   Group: iGEM07_UCSF   (2007-08-16)


YopH-EE under strong constitutive promotor

This device will express YopH, a pathogen derived irreversible inhibitor (phosphotase) of MapK Fus3 under the pAdh promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing the matching EE zipper. The effector is fused mCherry (RFP) on the N-terminal.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2123
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2123
    Illegal NotI site found at 2682
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2665
    Illegal XhoI site found at 1763
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2123
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2123
    Illegal NgoMIV site found at 476
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1126


[edit]
Categories
Parameters
None