Device

Part:BBa_I766671

Designed by: Eric Chou   Group: iGEM07_UCSF   (2007-08-16)


YopH-EE under weak constitutive promotor

This device will express YopH, a pathogen derived irreversible inhibitor (phosphotase) of MapK Fus3 under the pSte5 promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing the matching EE zipper. The effector is fused mCherry (RFP) on the N-terminal.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 345
    Illegal PstI site found at 1223
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1223
    Illegal NotI site found at 1782
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1765
    Illegal XhoI site found at 863
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 345
    Illegal PstI site found at 1223
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 345
    Illegal PstI site found at 1223
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None