Generator

Part:BBa_I766670

Designed by: Eric Chou   Group: iGEM07_UCSF   (2007-08-16)

YopH-EE under medium constitutive promotor

This device will express YopH, a pathogen derived irreversible inhibitor (phosphotase) of MapK Fus3 under the pCyc promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing the matching RR zipper. The effector is fused mCherry (RFP) on the N-terminal.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 720
    Illegal PstI site found at 2035
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2035
    Illegal NotI site found at 2594
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2577
    Illegal XhoI site found at 1675
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 720
    Illegal PstI site found at 2035
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 720
    Illegal PstI site found at 2035
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1225


[edit]
Categories
Parameters
None