Device

Part:BBa_I766669

Designed by: Jimmy Jie Min Huang   Group: iGEM07_UCSF   (2007-08-16)


YopJ-RR under weak constitutive promotor

This device will express YopJ, a pathogen derived irreversible inhibitor (acetylase) of MapKK Ste7 under the pSte5 promotor. The effector protein is fused to an RR leucine zipper and will be recruited to elements possessing the matching EE zipper. The effector is fused mCherry (RFP) on the N-terminal.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 93
    Illegal PstI site found at 1852
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1852
    Illegal NotI site found at 2411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 901
    Illegal BamHI site found at 742
    Illegal BamHI site found at 2394
    Illegal XhoI site found at 1492
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 93
    Illegal PstI site found at 1852
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 93
    Illegal PstI site found at 1852
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 670
    Illegal SapI.rc site found at 694


[edit]
Categories
Parameters
None