Generator

Part:BBa_I766655

Designed by: alexander ng   Group: iGEM07_UCSF   (2007-08-16)

YopJ-EE under medium constitutive promotor

This device will express YopJ, a pathogen derived irreversible inhibitor (acetylase) of MapKK Ste7 under the pCyc promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing a matching RR zipper. The effector is fused mCherry (RFP) on the N terminal. The protein is under control of a constitutive Cyc1 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 614
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 614
    Illegal NotI site found at 2054
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1270
    Illegal BamHI site found at 1111
    Illegal BamHI site found at 2037
    Illegal XhoI site found at 254
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 614
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 614
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1039
    Illegal SapI.rc site found at 1063


[edit]
Categories
Parameters
None