Generator

Part:BBa_I766380

Designed by: Michael Chen   Group: iGEM07_UCSF   (2007-10-25)

YopH-RR under inducible promotor

YopH-RR under Fig1 promoter

Usage and Biology

YopH is a bacterial protein tyrosine phosphorylase. It catalyzes the removal of the phosphate from the tyrosine side chain present in the MAPK activation loop, therefore inactivating the kinase in an reversible manner. Here, YopH is N-terminally fused to mCherry, and C-terminally to the synthetic leucine zipper RR; being expressed under control of a Fig1 promoter (induced by activation of the mating pathway).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2202
    Illegal PstI site found at 1370
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1370
    Illegal NotI site found at 3350
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3333
    Illegal XhoI site found at 1010
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2202
    Illegal PstI site found at 1370
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2202
    Illegal PstI site found at 1370
    Illegal NgoMIV site found at 172
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2707


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