Reporter

Part:BBa_I766346

Designed by: Nili Sommovilla   Group: iGEM07_UCSF   (2007-10-23)

ING2 PH - mCherry under medium constitutive promoter

expresses a RFP fusion ING2PH domain under the medium strength promoter pCyc

Usage and Biology

Construct is under control of pCyc, a medium level constitutive promoter.

Reporter RFP is fused to the ING2 Pleckstrin Homology (PH) domain. ING2 has specific binding affinity for Phosphotidylinositol 5-Phosphate (PI[5]P).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 637
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 637
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1270
    Illegal XhoI site found at 262
    Illegal XhoI site found at 277
    Illegal XhoI site found at 1002
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 637
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 637
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None