Generator

Part:BBa_I766228

Designed by: Nili Sommovilla   Group: iGEM07_UCSF   (2007-10-22)

HMTM1-EE under medium constitutive promoter

Usage and Biology

Construct is under control of pCyc, a medium level constitutive promoter.

EE is one half of the EE-RR leucine zipper interaction, which has a dissociation constant in the nanomolar range.

HMTM1 stands for Human Myotubularin(MTM) related protein 1. HMTM1 binds Phosphatidylinositol 3-Phosphate (PI[3]P) and Phospatidylinosotol 3,5-Phosphate (PI[3,5]P) as substrates and dephosphorylates them at the 3-Phosphate position.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 941
    Illegal EcoRI site found at 1639
    Illegal XbaI site found at 594
    Illegal PstI site found at 1990
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 941
    Illegal EcoRI site found at 1639
    Illegal PstI site found at 1990
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 941
    Illegal EcoRI site found at 1639
    Illegal BglII site found at 357
    Illegal BamHI site found at 2285
    Illegal XhoI site found at 254
    Illegal XhoI site found at 338
    Illegal XhoI site found at 540
    Illegal XhoI site found at 1724
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 941
    Illegal EcoRI site found at 1639
    Illegal XbaI site found at 594
    Illegal PstI site found at 1990
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 941
    Illegal EcoRI site found at 1639
    Illegal XbaI site found at 594
    Illegal PstI site found at 1990
  • 1000
    COMPATIBLE WITH RFC[1000]


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