Device

Part:BBa_I766226

Designed by: Nili Sommovilla   Group: iGEM07_UCSF   (2007-10-22)


p110alpha-EE under weak constitutive promoter

This device expresses p110alpha fused to an EE leucine zipper under the weak constitutive promoter Ste5.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2349
    Illegal XbaI site found at 93
    Illegal XbaI site found at 2353
    Illegal PstI site found at 3235
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2349
    Illegal PstI site found at 3235
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2349
    Illegal BglII site found at 1685
    Illegal BamHI site found at 4037
    Illegal XhoI site found at 611
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2349
    Illegal XbaI site found at 93
    Illegal XbaI site found at 2353
    Illegal PstI site found at 3235
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2349
    Illegal XbaI site found at 93
    Illegal XbaI site found at 2353
    Illegal PstI site found at 3235
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2104


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Categories
Parameters
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