Generator

Part:BBa_I766225

Designed by: Nili Sommovilla   Group: iGEM07_UCSF   (2007-10-22)

WMTM1-EE under weak constitutive promoter

Usage and Biology

Construct is under control of pSte5, a weak constitutive promoter.

EE is one half of the EE-RR leucine zipper interaction, which has a dissociation constant in the nanomolar range.

WMTM1 stands for Worm (C. Elegans) Myotubularin(MTM) related protein 1. WMTM1 binds Phosphatidylinositol 3-Phosphate (PI[3]P) and Phospatidylinosotol 3,5-Phosphate (PI[3,5]P) as substrates and dephosphorylates them at the 3-Phosphate position.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1167
    Illegal EcoRI site found at 1313
    Illegal XbaI site found at 93
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1167
    Illegal EcoRI site found at 1313
    Illegal NheI site found at 1608
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1167
    Illegal EcoRI site found at 1313
    Illegal BglII site found at 699
    Illegal BamHI site found at 2597
    Illegal XhoI site found at 611
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1167
    Illegal EcoRI site found at 1313
    Illegal XbaI site found at 93
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1167
    Illegal EcoRI site found at 1313
    Illegal XbaI site found at 93
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None