Part:BBa_I766224

WMTM1-EE under weak constitutive promoter

This device expresses the MTM1 phosphatase from C. Elegans fused to an EE leucine zipper with the weak constitutive promoter Ste5.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 4235
  Illegal EcoRI site found at 4381
  Illegal XbaI site found at 93
  Illegal XbaI site found at 3176
  Illegal XbaI site found at 7992
  Illegal PstI site found at 2753
  Illegal PstI site found at 7585
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 4235
  Illegal EcoRI site found at 4381
  Illegal NheI site found at 4676
  Illegal PstI site found at 2753
  Illegal PstI site found at 7585
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 4235
  Illegal EcoRI site found at 4381
  Illegal BglII site found at 3767
  Illegal BamHI site found at 5471
  Illegal BamHI site found at 8670
  Illegal BamHI site found at 9061
  Illegal XhoI site found at 611
  Illegal XhoI site found at 2952
  Illegal XhoI site found at 3685
  Illegal XhoI site found at 7776
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 4235
  Illegal EcoRI site found at 4381
  Illegal XbaI site found at 93
  Illegal XbaI site found at 3176
  Illegal XbaI site found at 7992
  Illegal PstI site found at 2753
  Illegal PstI site found at 7585
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 4235
  Illegal EcoRI site found at 4381
  Illegal XbaI site found at 93
  Illegal XbaI site found at 3176
  Illegal XbaI site found at 7992
  Illegal PstI site found at 2753
  Illegal PstI site found at 7585
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2359
  Illegal BsaI site found at 7191
  Illegal BsaI.rc site found at 3606
  Illegal BsaI.rc site found at 8422