Generator

Part:BBa_I766222

Designed by: Nili Sommovilla   Group: iGEM07_UCSF   (2007-10-22)

HMTM1-EE under weak constitutive promoter

Usage and Biology

Construct is under control of pSte5, a weak constitutive promoter.

EE is one half of the EE-RR leucine zipper interaction, which has a dissociation constant in the nanomolar range.

HMTM1 stands for Human Myotubularin(MTM) related protein 1. HMTM1 binds Phosphatidylinositol 3-Phosphate (PI[3]P) and Phospatidylinosotol 3,5-Phosphate (PI[3,5]P) as substrates and dephosphorylates them at the 3-Phosphate position.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1298
    Illegal EcoRI site found at 1996
    Illegal XbaI site found at 93
    Illegal XbaI site found at 951
    Illegal PstI site found at 2347
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1298
    Illegal EcoRI site found at 1996
    Illegal PstI site found at 2347
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1298
    Illegal EcoRI site found at 1996
    Illegal BglII site found at 714
    Illegal BamHI site found at 2642
    Illegal XhoI site found at 611
    Illegal XhoI site found at 695
    Illegal XhoI site found at 897
    Illegal XhoI site found at 2081
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1298
    Illegal EcoRI site found at 1996
    Illegal XbaI site found at 93
    Illegal XbaI site found at 951
    Illegal PstI site found at 2347
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1298
    Illegal EcoRI site found at 1996
    Illegal XbaI site found at 93
    Illegal XbaI site found at 951
    Illegal PstI site found at 2347
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None