Device

Part:BBa_I766221

Designed by: Nili Sommovilla   Group: iGEM07_UCSF   (2007-10-22)


p110alpha-EE under pGal1 promoter

device expresses p110alpha, a phosphatidyl inositol 3-kinase fused to an EE leucine zipper under a galactose inducible promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 195
    Illegal EcoRI site found at 2750
    Illegal XbaI site found at 2754
    Illegal PstI site found at 3636
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 195
    Illegal EcoRI site found at 2750
    Illegal PstI site found at 3636
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 195
    Illegal EcoRI site found at 2750
    Illegal BglII site found at 2086
    Illegal BamHI site found at 4438
    Illegal XhoI site found at 1012
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 195
    Illegal EcoRI site found at 2750
    Illegal XbaI site found at 2754
    Illegal PstI site found at 3636
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 195
    Illegal EcoRI site found at 2750
    Illegal XbaI site found at 2754
    Illegal PstI site found at 3636
    Illegal AgeI site found at 619
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2505


[edit]
Categories
Parameters
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