Deleted
Sample Discontinued
Experience: None
Not Used
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Device

Part:BBa_I766218

Designed by: Nili Sommovilla   Group: iGEM07_UCSF   (2007-10-22)

HMTM1-EE under pGAl1 promoter

Expresses Human MTM1 phosphatase fused to an EE leucine zipper (protein interaction domain, "EE" leucine zippers bind to "RR" leucine zippers) under the Gal1 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 209
    Illegal EcoRI site found at 1713
    Illegal EcoRI site found at 2411
    Illegal XbaI site found at 1366
    Illegal PstI site found at 2762
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 209
    Illegal EcoRI site found at 1713
    Illegal EcoRI site found at 2411
    Illegal PstI site found at 2762
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 209
    Illegal EcoRI site found at 1713
    Illegal EcoRI site found at 2411
    Illegal BglII site found at 1129
    Illegal BamHI site found at 3057
    Illegal XhoI site found at 1026
    Illegal XhoI site found at 1110
    Illegal XhoI site found at 1312
    Illegal XhoI site found at 2496
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 209
    Illegal EcoRI site found at 1713
    Illegal EcoRI site found at 2411
    Illegal XbaI site found at 1366
    Illegal PstI site found at 2762
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 209
    Illegal EcoRI site found at 1713
    Illegal EcoRI site found at 2411
    Illegal XbaI site found at 1366
    Illegal PstI site found at 2762
    Illegal AgeI site found at 633
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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