Generator

Part:BBa_I766212

Designed by: Nili Sommovilla   Group: iGEM07_UCSF   (2007-10-22)

Ste2-GFP-HMTM1 under endogenous promoter

Usage and Biology

Ste2 is a native to the yeast Saccharomyces cerevisiae, and is an integral membrane receptor for the mating pheromone alpha factor. Upon induction with alpha factor, Ste2 is endocytosed into the cell, and eventually ends up in the vacuole.

HMTM1 stands for Human Myotubularin(MTM) related protein 1. HMTM1 binds Phosphatidylinositol 3-Phosphate (PI[3]P) and Phospatidylinosotol 3,5-Phosphate (PI[3,5]P) as substrates and dephosphorylates them at the 3-Phosphate position.

This part has been tested, and we believe that Ste2 is being mislocalized and is not functional in the native pathway. In addition, its phenotype exhibits a much larger vacuole as compared to wild type yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3736
    Illegal EcoRI site found at 4434
    Illegal XbaI site found at 2546
    Illegal XbaI site found at 3389
    Illegal XbaI site found at 7407
    Illegal PstI site found at 2123
    Illegal PstI site found at 4785
    Illegal PstI site found at 7000
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3736
    Illegal EcoRI site found at 4434
    Illegal PstI site found at 2123
    Illegal PstI site found at 4785
    Illegal PstI site found at 7000
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3736
    Illegal EcoRI site found at 4434
    Illegal BglII site found at 3152
    Illegal BamHI site found at 4886
    Illegal BamHI site found at 8085
    Illegal XhoI site found at 2322
    Illegal XhoI site found at 3055
    Illegal XhoI site found at 3133
    Illegal XhoI site found at 3335
    Illegal XhoI site found at 4519
    Illegal XhoI site found at 7191
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3736
    Illegal EcoRI site found at 4434
    Illegal XbaI site found at 2546
    Illegal XbaI site found at 3389
    Illegal XbaI site found at 7407
    Illegal PstI site found at 2123
    Illegal PstI site found at 4785
    Illegal PstI site found at 7000
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3736
    Illegal EcoRI site found at 4434
    Illegal XbaI site found at 2546
    Illegal XbaI site found at 3389
    Illegal XbaI site found at 7407
    Illegal PstI site found at 2123
    Illegal PstI site found at 4785
    Illegal PstI site found at 7000
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1729
    Illegal BsaI site found at 6606
    Illegal BsaI.rc site found at 2976
    Illegal BsaI.rc site found at 7837


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