Generator

Part:BBa_I766019

Designed by: Michael Chen   Group: iGEM07_UCSF   (2007-10-25)

Ste2-GFP-RR under endogenous promoter


Usage and Biology

Ste2 is a native to the yeast Saccharomyces cerevisiae, and is an integral membrane receptor for the mating pheromone alpha factor. Upon induction with alpha factor, Ste2 is endocytosed into the cell, and eventually ends up in the vacuole.

RR is one half of the EE-RR leucine zipper interaction. This interaction has a dissociation constant in the nanomolar range.

This part has been tested, and it has been shown that this construct retains the functionality of native Ste2. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2546
    Illegal XbaI site found at 5763
    Illegal PstI site found at 2123
    Illegal PstI site found at 5356
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2123
    Illegal PstI site found at 5356
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3242
    Illegal BamHI site found at 6441
    Illegal XhoI site found at 2322
    Illegal XhoI site found at 3055
    Illegal XhoI site found at 5547
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2546
    Illegal XbaI site found at 5763
    Illegal PstI site found at 2123
    Illegal PstI site found at 5356
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2546
    Illegal XbaI site found at 5763
    Illegal PstI site found at 2123
    Illegal PstI site found at 5356
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1729
    Illegal BsaI site found at 4962
    Illegal BsaI.rc site found at 2976
    Illegal BsaI.rc site found at 6193


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