Signalling

Part:BBa_I765013

Designed by: Juliana Maria Navia and Alex Quintero   Group: iGEM07_Colombia_Israel   (2007-10-26)


Iron promoter- luxI HSL signaling device

LuxI produce 3OC6HSL, which binds to LuxR and activate the Lux R promoter. It also has The right promoter of the lux cassett wich gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 160
    Illegal PstI site found at 712
    Illegal PstI site found at 940
    Illegal PstI site found at 961
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 160
    Illegal PstI site found at 712
    Illegal PstI site found at 940
    Illegal PstI site found at 961
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1707
    Illegal XhoI site found at 814
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 160
    Illegal PstI site found at 712
    Illegal PstI site found at 940
    Illegal PstI site found at 961
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 160
    Illegal PstI site found at 712
    Illegal PstI site found at 940
    Illegal PstI site found at 961
    Illegal NgoMIV site found at 47
    Illegal NgoMIV site found at 397
    Illegal NgoMIV site found at 511
    Illegal NgoMIV site found at 565
    Illegal NgoMIV site found at 868
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 421
    Illegal BsaI.rc site found at 1856


[edit]
Categories
Parameters
None