Device

Part:BBa_I763028

Designed by: Alice Pasini   Group: iGEM07_Bologna   (2007-09-06)


pLac-cI-LacY Tester

In absence of glucose and in presence of IPTG, pLac is activated and switch the trascription of the Lactose permase (Lac Y) gene and of cI repressor gene with GFP(+LVA) as a reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3196
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4363


[edit]
Categories
Parameters
None