Device

Part:BBa_I763027

Designed by: Alice Pasini   Group: iGEM07_Bologna   (2007-09-06)


pLac activity Tester repressed by LacI

In absence of enough endogenous LacI repressor protein, PLac is not repressed and GFP is expressed. LacI expression regulated by pTetR allows to avoid this unregulated activity of Plac Promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2248


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Categories
Parameters
None