Measurement
PLac-CI-La

Part:BBa_I763019

Designed by: Alice Pasini   Group: iGEM07_Bologna   (2007-08-21)

CI and LacY coding device switched on by IPTG with GFP as a reporter

PLac activation induces the expression of cI repressor protein and of the Lactose permease (Lac Y) gene with GFP (+LVA tag) as a reporter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1818
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2985


[edit]
Categories
Parameters
None