Deleted
Sample Discontinued
Experience: None
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Measurement
PLac-CI-La

Part:BBa_I763018

Designed by: Alice Pasini   Group: iGEM07_Bologna   (2007-08-20)


GFP, LacI, LacY and CI coding device switched on by IPTG

PLac activation induces the expression of CI reppressor protein, Lactose permase (Lac Y) gene, LacI repressor protein with GFP (+LVA tag) as a reporter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3488
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1017
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4164


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Parameters
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