Intermediate

Part:BBa_I761015

Designed by: 2007 NYMU_Taiwan iGEM Team   Group: iGEM07_Taipei   (2007-10-26)


B0034:I761004

Twin arginin signal peptide is an efficient protein export signal sequence of E. coli. The purpose of this construct is to allow E. coli export mouse insulin degradation enzyme(IDE) automatically, allowing controlled insulin degradation of extracellular insulin.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 783
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1944
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2483
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 783
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 783
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1392
    Illegal SapI.rc site found at 823
    Illegal SapI.rc site found at 991


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Categories
Parameters
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