Generator

Part:BBa_I761006

Designed by: 2007 NYMU_Taiwan iGEM Team   Group: iGEM07_Taipei   (2007-10-19)


Glucose and cinr+cinl controlled mouse insulin degradation enzyme generator

This generator is under control of extracellular glucose concentration and insulin activation state. High extracellular glucose concentration will turn off the generator by the binding of phosphorylated OmpR. Unless BBa_I761007 has respond to prior high extracellular glucose concentration, this generator will not turn on. This is achieved by the control of cinr and cinl. After mouse insulin degradation enzyme is generated, it will be exported and can degrade extracellular insulin.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1086
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2786
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1086
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1086
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1695
    Illegal SapI.rc site found at 1126
    Illegal SapI.rc site found at 1294


[edit]
Categories
Parameters
None