Coding

Part:BBa_I761004

Designed by: 2007 NYMU_Taiwan iGEM Team   Group: iGEM07_Taipei   (2007-10-19)


TAT signal+IDE

Twin arginin signal peptide is an efficient protein export signal sequence of E. coli. The purpose of this construct is to allow E. coli export mouse insulin degradation enzyme(IDE) automatically, allowing controlled insulin degradation of extracellular insulin.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 765
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1926
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2465
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 765
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 765
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1374
    Illegal SapI.rc site found at 805
    Illegal SapI.rc site found at 973


[edit]
Categories
Parameters
None