Project

Part:BBa_I759031

Designed by: Josh Michener   Group: iGEM07_Caltech   (2007-10-24)

Constitutive cro

The pTet promoter, in the absence of tet repressor, produces cro transcripts. As the transcript now contains a terminator, the cell will translate the transcript into Cro protein. Because the construct does not contain any tetR producing elements, this construct will result in the maximum concentration of Cro protein production (per copy of the gene) in the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None