Composite

Part:BBa_I759021

Designed by: Kat Pak   Group: iGEM07_Caltech   (2007-10-22)


pBAD-spacer

This is the standard sequence into which the trans-activating sequences were inserted by replacing the overlap region.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1449
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1449
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1383
    Illegal XhoI site found at 1442
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1449
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1449
    Illegal NgoMIV site found at 92
    Illegal AgeI site found at 1218
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1200


[edit]
Categories
Parameters
None