Coding

Part:BBa_I757003

Designed by: iGEM Team Freiburg 2007   Group: iGEM07_Freiburg   (2007-10-24)

xCaM_rCKK_xCaM_fusionpart

  1. calmodulin: undergoes a strong conformational change upon binding calcium
  2. purpose: design of Ca2+ sensors
  3. this module comprises xenopus N-terminal part of CaM, rat CKK binding region, xenopus C-terminal CaM
  4. backtranslated from the design of K. Truong et al. Methods Mol Biol. 2007;352:71-82, Nat Struct Biol. 2001 Dec;8(12):1069-73.
  5. NgoMIV / AgeI protein fusion part
  6. iGEM Team Freiburg 2007
  7. Synthetic DNA by GeneArt optimized for E.coli
  8. Part in pGA4 vector (AmpR, ColEI ori)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 544
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None